International Journal of Molecular Biology and Biochemistry

International Journal of Molecular Biology and Biochemistry






Online ISSN: 2664-651X
Print ISSN: 2664-6501


International Journal of Molecular Biology and Biochemistry
International Journal of Molecular Biology and Biochemistry
2019, Vol. 1, Issue 2
In present study efforts were made to improve to study invitro callus induction of safflower. Safflower local genotype was used to study in vitro callus induction from leaf explant. Safflower seed were in vitro germinated on plane MS media. The leaf explants were excised from it aseptically cultured on MS media with different concentrations of 2, 4-D and incubated at 25 ± 2°C in light condition. Good callusing was observed on MS media containing 3.0, 3.5, 4.0, mg / l 2, 4-D within 3-4 week, yielding white embryonic and globular shaped calli. Profuse callusing was obtained on MS media containing 3.5 mg / l 2, 4-D.
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