International Journal of Molecular Biology and Biochemistry
Vol. 3, Issue 1, Part A (2021)
Bioinformatics approaches for cross-species liver cancer analysis based on single-cell RNA sequencing data in rats mice and human insights causative genes and pathways
In this research, we first recognized 2322 differentially expressed genes via way of means of evaluating gene expression profiles for 2 control, adenoma, and carcinoma samples by the use of an F-test. These genes have been sooner or later mapped to the rat chromosomes through the use of a unique visualization tool, the Chromosome Plot. Using the equal plot, we similarly mapped the enormous genes to orthologous chromosomal places in people and mice. Several genes expressed in rat 1q which is probably amplified in rat liver maximum cancers map to the human chromosomes 10, 11, and 19 and to the mice chromosomes 7, 17, and 19, which have been implicated in studies of human and mice liver cancers. Using Comparative Genomics Single cell RNA sequencing data Analysis (CGMA), we recognized areas of capacity aberrations in people. Lastly, a pathway evaluation became performed to expect altered human pathways primarily based totally on statistical evaluation and extrapolation from the rat records. All of the recognized pathways were regarded to be vital with-inside the etiology of the human liver most cancers, inclusive of molecular cycle control, molecular boom and differentiation, apoptosis, transcriptional regulation, and protein metabolism. Background: The final touch of the sequencing of the human, mouse, and rat genomes and understanding of cross-species gene homologies permit research of differential gene expression in animal fashions. These styles of research have the capacity to substantially beautify our information of sicknesses inclusive of liver most cancers in people. Genes co-expressed throughout a couple of species are maximum in all likelihood to have conserved features. We have used numerous bioinformatics methods to take a look at single cell RNA sequencing data expression profiles from liver neoplasms that stand up in albumin-SV40 transgenic rats to explain genes, chromosome aberrations, and pathways that are probably related to human liver most cancers. Aim and objectives: The main aim of this research study was to evaluate the bioinformatics approaches of liver cancer with single cell RNA sequencing Data. This study will analyze the role of RNA changes that would help in liver cancer analysis. Another aim was to evaluate and differentiate the pattern on liver cancer in rats, mice and humans.
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Sudheer Menon. Bioinformatics approaches for cross-species liver cancer analysis based on single-cell RNA sequencing data in rats mice and human insights causative genes and pathways. Int. J. Mol. Biol. Biochem. 2021;3(1):05-13.