Vol. 5, Issue 2, Part A (2023)

The overuse and abuse of antibiotics have been accelerated antibiotic resistance, to solve this problem, it was found many insect species have potential antimicrobial properties against wide range of resistant pathogens. Through sequential extraction method by acidic methanol, chloroform and hexane solvents, insect body extract of Gryllotalpa gryllotalpa and grubs of Pentodon algerinum besides feces of Gypsonoma euphraticana larvae were tested against Gram positives Bacillus cereus, Bacillus coagulans and Staphylococcus aureus and Gram negatives Salmonella typhi, Escherichia coli and Klebsiella pneumoniae. The antibiotics Ceftriaxone (CRO) and Ampicillin (AM) were used as standard drugs. The antibacterial growth inhibition was estimated by well diffusion method. High significant antibacterial activity against the tested bacteria by acidic methanol then chloroform extracts, while hexane extract of all the three insect species only caused significant growth inhibition of Staph. aureus. Also, it was found growth inhibition 20.0 mm or more were induced by: Acidic meOH extracts of G. gryllotalpa and P algerinum for S. typhi and E. coli, besides chloroform G. gryllotalpa extract for S. typhi. The tested bacteria Staph. aureus, S. typhi, and K. pneumoniae were resisted AM standard drug, while E. coli resistant to both AM and CRO antibiotics. Acidic meOH and chloroform body extract of G. gryllotalpa and P algerinum have bioactive compounds with promising antibacterial properties, for confrontation overcoming antibiotic resistance.