Vol. 6, Issue 1, Part A (2024)

MicroRNAs are non-coding RNAs that play a pivotal role in gene expression. The regulatory role of miRNA for genes illustrates its involvement in cancer and many other infectious diseases. miRNAs regulate a single gene or multiple genes, which propose their regulatory mechanisms in diagnosing and treating many diseases. Therefore, miRNAs may act as tumor suppressors or oncogenes based on their targeted genes and the specific pathway for their regulation. The miRNA-141, as a member of the miRNA 200 family, plays a vital role in tumorigenesis. As in liver cancer cells, miRNA-141 can target the STAT4 gene expression, which inhibits its proliferation and migration. In this study, we try via In Silico analysis to identify the potential targeted genes for miRNA-141 using many In Silico tools and bioinformatics software like PicTar software to unleash the molecular role of miRNA-141 in human carcinoma tumorigenesis. Interestingly, the most identified targets with low required energy and high PicTar score include a variety of tumor and metastasis suppressor genes such as WTAP as a tumor suppressor gene. The deleted liver cancer -1 (DLC-1) that is regulated by miRNA141 and CHES1 which expresses the forkhead transcription factor checkpoint suppressor 1 CHES1 is reduced in many types of cancers. The deficient energy required for targeting these genes and completing the interfering reaction makes the data presented here a straightforward and spontaneous cellular event.